About us
Espace utilisateur
Education
INSTN offers more than 40 diplomas from operator level to post-graduate degree level. 30% of our students are international students.
Professionnal development
Professionnal development
Find a training course
INSTN delivers off-the-self or tailor-made training courses to support the operational excellence of your talents.
Human capital solutions
At INSTN, we are committed to providing our partners with the best human capital solutions to develop and deliver safe & sustainable projects.
Thesis
Home   /   Thesis   /   Design of a new light-sheet microscope for the temporal monitoring of organoids-on-chip

Design of a new light-sheet microscope for the temporal monitoring of organoids-on-chip

Engineering sciences Health and environment technologies, medical devices Instrumentation Technological challenges

Abstract

The subject of the thesis is the development of a fluorescence light-sheet microscope for the optical characterization of organoids-on-chips and 3D organoids. The thesis will focus on the conception of a compact multi-color 3D system, to allow time-lapse imaging of multi-scattering 3D samples directly in a cell culture incubator. The work will begin with a clear understanding of the miniaturization process on the quality of the images. The excitation will be correctly model to avoid optical artefacts and to allow the deepest penetration into the biological tissue. The candidate will be responsible to test different optical strategies as well as different excitation wavelengths. As a final step, the system will be characterized in a cell culture incubator for the morphological and functional monitoring of organs-on-a-chip and 3D organoids using specific fluorescent markers. If needed, novel modifications of the microfluidic chamber with integrated optical functions will be proposed. The research program will mainly focus on the morphological and functional monitoring of two samples: pancreatic organoids on a microfluidic chip and 3D brain organoids.

Laboratory

Département Microtechnologies pour la Biologie et la Santé (LETI) (inactif)
Archive_Service des sYstèmes de Mesure pour la Santé
Laboratoire Instrum optique et traitement de l’Info pour l'Imagerie In Vitro
Université Grenoble Alpes
Top envelopegraduation-hatlicensebookuserusersmap-markercalendar-fullbubblecrossmenuarrow-down